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中文名称

小鼠孤雄单倍体胚胎干细胞系（稳定表达Cas9）,IG△DMR-H19△DMR-AGH-C9,SHBCC E00331

英文名称

IG△DMR-H19△DMR-AGH-C9

细胞描述

稳定表达Cas9的DKO (IG△DMR- H19△DMR -AGH) 孤雄单倍体胚胎干细胞系【本细胞只提供冻存管】。需定期通过流式分选富集单倍体细胞，详见《细胞说明书》。Fee: ￥3000 (domestic); 800 USD (abroad). Note: A transmittal fee is required to reimburse the Stem Cell Bank for cell collection, preservation, preparation and paper han

动物种类

Mus musculus, mouse; C57 stain

性别

Male

组织来源

Tissue: Inner cell mass; Cell Type: Haploid embryonic stem cell

形态

Spherical colony

培养基

ES-DMEM(Invitrogen,12430) 400 ml

ES-FBS(Gibco,16141-079) 75 ml

NEAA(Invitrogen,11140) 5 ml

Nucleosides（Millipore,ES-008-D） 5 ml

Pen-Strep（Millipore,TMS-AB2-C） 5 ml

Glutamax（invitrogen 35050） 5 ml

参考文献

Zhong, C., Yin, Q., Xie, Z., Bai, M., Dong, R., Tang, W., Xing, Y.H., Zhang, H., Yang, S., Chen, L.L., et al. (2015). CRISPR-Cas9-Mediated Genetic Screening in Mice with Haploid Embryonic Stem Cells Carrying a Guide RNA Library. Cell Stem Cell 17, 221-232

备注

服务费

3400