平台资源号：

菌株保藏编号：SHMCC D72249

中文名称：腾冲热互养菌SHMCC D72249=JCM 17260

拉丁名称：Thermosyntropha tengcongensis

模式菌株：模式菌株

其它保藏中心编号：=JCM 17260

来源历史：咨询客服

收藏时间：

参考用途：模式菌株

生物危害程度：四类

致病对象：

培养温度：60℃

需氧类型：

提供方式：冻干物

保存温度：冷藏4-10℃

THERMOSYNTROPHA L-60 MEDIUM
0977 THERMOSYNTROPHA L-60 MEDIUMSolution A:KH2PO4  0.54g Na2HPO4  1.33g Yeast extract (BD-Difco)  3.0g Tryptone (BD-Difco)  0.5g Bacto peptone (BD-Difco)  1.5g Resazurin  1.0mg Distilled water  760.0ml Solution B:NH4Cl  0.3g NaCl  0.3g CaCl2·2H2O  0.01g MgCl2·6H2O  0.1g Trace element solution (见培养基0901） 1.0ml Distilled water  100.0ml Solution C:Crotonic acid  1.72g Distilled water  100.0ml Adjust pH to 7.0Autoclave Solutions A B and C separately. After cooling aseptically and anaerobically combine the three solutions under a stream of N2-CO2 (80:20 v/v) then add the following solutions (filter-sterilized):Trace vitamins见培养基0901 10.0ml 8% NaHCO3 solution  30.0ml Prior to inoculation reduce the medium with 0.025% L-cysteine·HCl·H2O and 0.025% Na2S·9H2O (use 5% solutions autoclaved and stored under a N2 atmosphere).

参考文献内容