平台资源号：

菌株保藏编号：SHMCC D72269

中文名称：疮疱丙酸杆菌SHMCC D72269

拉丁名称：Propionibacterium acnes

模式菌株：非模式菌株

其它保藏中心编号：

来源历史：咨询客服

收藏时间：

参考用途：

生物危害程度：四类

致病对象：

培养温度：30℃

需氧类型：

提供方式：冻干物

保存温度：冷藏4-10℃

0989KH2PO4 0.3gK2HPO4 0.3g(NH4)2SO4 0.3gNaCl 0.6gMgSO4·7H2O 0.13gFeSO4·7H2O 2.0mgCaCl2·2H2O 8.0mgYeast extract (Difco) 2.0gTrypticase peptone (BBL) 2.0gSodium acetate 2.5gSodium formate 2.5gTrace minerals (见培养基0893) 10.0mlTrace vitamins (见培养基0901) 10.0mlNaHCO3 5.0gNa2S·9H2O 0.5gL-Cysteine·HCl·H2O 0.5gResazurin 1.0mgDistilled water 1.0LMix ingredients except NaHCO3 L-Cysteine·HCl·H2O and Na2S·9H2O and bring to a boil for 5-10 sec. Cool down under a stream of H2-CO2 (80:20) and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g. 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20) and seal with butyl rubber stoppers. Separately autoclave cysteine·HCl and Na2S·9H2O as 5% solutions respectively under a N2 atmosphere. Prior to inoculation aseptically and anaerobically add the cysteine·HCl and Na2S·9H2O solutions. Pressurize the inoculated vessels to 200 kPa H2-CO2 (80:20).

参考文献内容