平台资源号：

菌株保藏编号：SHMCC D72279

中文名称：厦门食热菌SHMCC D72279=DSM 19012

拉丁名称：Thermophagus xiamenensis

模式菌株：模式菌株

其它保藏中心编号：=DSM 19012

来源历史：咨询客服

收藏时间：

参考用途：模式菌株

生物危害程度：四类

致病对象：

培养温度：50℃

需氧类型：

提供方式：冻干物

保存温度：冷藏4-10℃

ANAEROBIC SEAWATER MEDIUM
1063 ANAEROBIC SEAWATER MEDIUMSolution A:KH2PO40.2g NH4Cl0.25g NaCl20.0g MgCl2 . 6 H2O3.0gKCl0.5gCaCl2 . 2 H2O0.15g Na-resazurin solution (0.1% w/v)0.5ml蒸馏水 940.0ml Solution B:Trace element solution SL-101.0mlSolution C:Vitamin solution1.0mlSolution D:Selenite-tungstate solution1.0mlSolution E:NaHCO3 3.5g蒸馏水50.0mlSolution F:D-glucose2.0gYeast extract0.5g蒸馏水1.0LSolution G:Na2S·9H2O0.3g蒸馏水10.0mlSparge solution A with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions B D and G are autoclaved separately under 100% N2 gas. Solutions C and F are prepared under 100% N2 gas and sterilized by filtration. Solution E is autoclaved under 80% N2 and 20% CO2 gas atmosphere. To complete the medium appropriate amounts of solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.2 - 7.4 if necessary.Selenite-tungstate solution:NaOH0.5gNa2SeO3·5 H2O3.0mgNa2WO4·2 H2O4.0mg蒸馏水1.0LTrace element solution SL-10：HCl (25%; 7.7 M) 10.0ml FeCl2·4 H2O1.5gZnCl270.0mgMnCl2·4 H2O100.0mgH3BO36.0mgCoCl2·6 H2O190.0mgCuCl2 ·2 H2O2.0mgNiCl2·6H2O24.0mgNa2MoO4·2H2O36.0mg蒸馏水990.0mlFirst dissolve FeCl2 in the HCl then dilute in water add and dissolve the other salts. Finally make up to 1000.0 ml.Seven vitamins solution：Vitamin B12100.0mgp-Aminobenzoic acid80.0mgD(+)-Biotin20.0mgNicotinic acid200.0mgCalcium pantothenate100.0mgPyridoxine hydrochloride300.0mgThiamine-HCl x· 2 H2O200.0mg蒸馏水1.0L

参考文献内容