平台资源号：

菌株保藏编号：SHMCC D72289

中文名称：栖酒窖共养单胞菌SHMCC D72289=JCM 13582

拉丁名称：Syntrophomonas cellicola

模式菌株：模式菌株

其它保藏中心编号：=JCM 13582

来源历史：咨询客服

收藏时间：

参考用途：模式菌株

生物危害程度：四类

致病对象：

培养温度：37℃

需氧类型：

提供方式：冻干物

保存温度：冷藏4-10℃

1064Solution A:Trypticase peptone (BD BBL)1.0g Mineral solution50.0mlTrace element solution SL-101.0mlRumen fluid clarified50.0mlButyric acid1.7gNa2SO42.8 gNa-resazurin solution (0.1% w/v)0.5ml蒸馏水 830.0ml Solution B:NaHCO3 3.5g蒸馏水50.0mlSolution C:Vitamin solution1.0mlSolution D:L-Cysteine-HCl·H2O0.3g蒸馏水10.0mlSolution E:Na2S·9H2O0.3g蒸馏水10.0mlAdd and dissolve ingredients of solution AMineral solution：KH2PO410.0gMgCl2·6 H2O6.6gNaCl8.0g NH4Cl8.0gCaCl2·2 H2O1.0g蒸馏水1.0LTrace element solution SL-10：HCl (25%; 7.7 M) 10.0ml FeCl2·4 H2O1.5gZnCl270.0mgMnCl2·4 H2O100.0mgH3BO36.0mgCoCl2·6 H2O190.0mgCuCl2 ·2 H2O2.0mgNiCl2·6H2O24.0mgNa2MoO4·2H2O36.0mg蒸馏水990.0mlFirst dissolve FeCl2 in the HCl then dilute in water add and dissolve the other salts. Finally make up to 1000.0 ml.Clarified rumen fluid:Rumen fluid from cow or sheep is filtered through muslin autoclaved at 121°C for 15 min and then centrifuged at 27000g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C.Seven vitamins solution：Vitamin B12100.0mgp-Aminobenzoic acid80.0mgD(+)-Biotin20.0mgNicotinic acid200.0mgCalcium pantothenate100.0mgPyridoxine hydrochloride300.0mgThiamine-HCl x· 2 H2O200.0mg蒸馏水1.0L

参考文献内容