平台资源号：

菌株保藏编号：SHMCC D72324

中文名称：氧化巴豆酸嗜碱菌SHMCC D72324=JCM 11672

拉丁名称：Alkaliphilus crotonatoxidans

模式菌株：模式菌株

其它保藏中心编号：=JCM 11672

来源历史：咨询客服

收藏时间：

参考用途：模式菌株

生物危害程度：四类

致病对象：

培养温度：37℃

需氧类型：

提供方式：冻干物

保存温度：冷藏4-10℃

ALKALIPHILUS CROTONATOXIDANS MEDIUM
Trace metal solution: 1058 ALKALIPHILUS CROTONATOXIDANS MEDIUMYeast extract (BD-Difco)  2.0g Tryptone (BD-Difco)  2.0g Crotonic acid  0.86g KH2PO4 0.5g MgCl2·6H2O  0.33g NaCl  0.4g NH4Cl  0.4g CaCl2·2H2O  0.05g Trace metal solution (see below)  10.0ml Trace vitamins (见培养基0901)  10.0ml NaHCO3  3.5g L-Cysteine·HCL·H2O  0.5g Na2S·9H2O  0.5g Resazurin  1.0mg Dissolve ingredients except crotonic acid NaHCO3 L-cysteine·HCl·H2O and Na2S·9H2O in 900 ml of distilled water and adjust pH to 7.0-7.5. Bring to a boil for 5-10 sec then cool down under a stream of N2-CO2 (80:20 v/v) and add NaHCO3 to the medium. Dispense 0.9 volume of the medium into suitable culture vessels under a stream of N2-CO2 (80:20 v/v) and seal with butyl rubber stoppers. After autoclaving stand the medium overnight. Add 0.1 volume of a filter-sterilized crotonic acid solution (0.86 g/100 ml pH 7.0) to the medium. Autoclave cysteine·HCl and Na2S·9H2O as 5% solutions respectively under a N2 atmosphere. Prior to inoculation aseptically and anaerobically add the solutions of crotonic acid L-cysteine·HCl·H2O and Na2S·9H2O to the medium.MnSO4·xH2O  1.0g Fe(NH4)2(SO4)2·6H2O  0.8g CoCl2·6H2O  0.2g ZnSO4·7H2O  0.2g CuCl2·2H2O  0.02g NiCl2·6H2O  0.02g Na2MoO4·2H2O  0.02g Na2SeO4  0.02g Na2WO4  0.02g Nitrilotriacetic acid  2.0g Distilled water  1.0L Adjust pH to 6.0 with KOH.

参考文献内容