平台资源号：

菌株保藏编号：SHMCC D72830

中文名称：蚕豆根瘤菌SHMCC D72830

拉丁名称：Rhizobium fabae

模式菌株：非模式菌株

其它保藏中心编号：

来源历史：咨询客服

收藏时间：

参考用途：

生物危害程度：四类

致病对象：

培养温度：37℃

需氧类型：

提供方式：冻干物

保存温度：冷藏4-10℃

酵母粉甘露醇琼脂
0454 酵母粉甘露醇琼脂 K2HPO40.5gMgSO4·7H2O0.2g NaCl0.1gMannitol10.0gYeast Extract (BD 212750)0.4g Wolfe's Mineral Solution (see below)0.01LDistilled water to1.0L Autoclave at 121C for 15 minutes.Wolfe's Mineral Solution:Available from ATCC as a sterile ready-to-use liquid (Trace MineralSupplement catalog no. MD-TMS.)Nitrilotriacetic acid1.5gMgSO4· 7H2O3.0gMnSO4· H2O0.5 gNaCl1.0gFeSO4 · 7H2O0.1gCoCl2 ·6H2O0.1gCaCl20.1gZnSO4· 7H2O0.1gCuSO4· 5H2O0.01gAlK(SO4)2·12H2O0.01gH3BO30.01gNa2MoO4· 2H2O0.01gDistilled water1.0LAdd nitrilotriacetic acid to approximately 500 ml of water and adjust to pH 6.5 with KOH to dissolve the compound. Bring volume to 1.0 L with remaining water and add remaining compounds one at a time.

参考文献内容