平台资源号：

菌株保藏编号：SHMCC D80432

中文名称：深红红螺菌

拉丁名称：Rhodospirillumrubrum

模式菌株：非模式菌株

其它保藏中心编号：DSM50914=ATCC27048=ICPB2204

来源历史：咨询客服

收藏时间：

参考用途：宿主细胞、生物应用

生物危害程度：四类

致病对象：

培养温度：30℃

需氧类型：厌氧，光照

提供方式：冻干粉

保存温度：

红螺菌培养基
酵母提取物 0.30 g 乙醇 0.50 ml 琥珀酸钠 1.00 g 乙酸铵 0.50 g 柠檬酸铁(0.1% in H2O) 5.00 ml KH2PO4 0.50 g MgSO4x 7 H2O 0.40 g NaCl 　0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g 维生素B12 组份(10 mg in 100 ml H2O) 0.40 ml 微量元素组份 SL-6 (如下) 1.00 ml 蒸馏水 　1050.00 ml pH 6.8. Boil the medium under a stream of nitrogen gas for few minutes and distribute 45 ml medium into 50 ml screw-capped bottles (already flushed with nitrogen gas). Bubble each bottle with nitrogen gas and close immediately with a rubber septum and screw tight. Autoclave at 121°C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp. For brown and other oxygen sensitive Rhodospirillaceae add 300 mg of L-cysteine (0.03% end concentration) to the boiling medium and readjust the pH to 6.8 or to the prepared medium in bottles inject neutralized sulfide solution (0.005 to 0.01% end concentration). The medium has been modified according to reference 3365. 微量元素组份SL-6: ZnSO4 x 7 H2O 　0.10 g MnCl2 x 4 H2O 0.03 g H3BO3 0.30 g CoCl2 x 6 H2O 0.20 g CuCl2 x 2 H2O 0.01 g NiCl2 x 6 H2O 　0.02 g Na2MoO4 x 2 H2O 0.03 g 蒸馏水 1000.00 ml

参考文献内容