平台资源号：

菌株保藏编号：SHMCC D83050

中文名称：Corallococcusllansteffanensis

拉丁名称：Corallococcusllansteffanensis

模式菌株：模式菌株

其它保藏中心编号：NBRC114100=DSM108844

来源历史：咨询客服

收藏时间：

参考用途：科研

生物危害程度：四类

致病对象：

培养温度：30℃

需氧类型：好氧

提供方式：冻干粉

保存温度：

VY/2 AGAR
Baker's yeast 5.00 g Vitamin B12 0.50 mg CaCl2 x 2 H2O 1.36 g Agar (Difco) 15.00 g Distilled water 1000.00 ml Sterilize vitamin B12 separately by filtration. Prepare and store yeast cells as autoclaved stock suspension (5 g baker's yeast/100 ml distilled water, adjust pH to 6.5 and autoclave). Adjust pH of medium to 7.2 with KOH before, and after autoclaving and cooling to 50°C (use pH-indicator paper). For suspension of freeze-dried cells from ampoules add about 0.5 - 1.0 ml medium MD1 (per liter: casiton 3.0 g; calciumchloride dihydrate 0.7 g; magnesiumsulphate heptahydrate 2.0 g) to the vial with freeze dried material. For DSM 11116 reduce amount of vitamin B12 to 0.05 mg/l.

参考文献内容